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Objective To investigate the relationship between the indexes change of lactate dehydrogenase(LDH),serum tumor associated material(BXTM),β2-microglobulin(β2-MG),C-reactive protein(CRP) and D-dimer(DD) with lymphoma cell bone marrow infiltration(BMI) degree in the patients with non-Hodgkin's lymphoma(NHL).Methods Seventy-four cases of initially treated NHL were retrospectively analyzed and divided into the bone marrow invasion group(BMI,26 cases),lymphoma cell leukemia group(LMCL,31 cases) and non-BMI group(N-BMI,17 cases).The related indexes levels in each group were detected.ROC curve was drawn and analyzed.Results The levels of LDH,β2-MG,CRP and DD levels in the BMI group and LMCL group were higher than those in the N-BMI group(P＜0.05).Based on the ROC curve analysis,the optimal diagnostic cut-off points of each index in BMI were determined.The CRP and LDH had good sensitivity(100.00 ％),specificity(80.00 ％-100.00 ％) and diagnostic accuracy(80.00 ％ 100.00％).In the LMCL group,the diagnostic accuracy of DD,BXTM and β2-MG was 63.30 ％-100.00％.Conclusion The changes of LDH,CRP,β2-MG and DD in the patients with NHL are consistent with the BMI severity of lymphoma cells,which has a good clinical value in staging,curative effect judgment and prognosis.
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Objective:To investigate the expression of IL-6 and STAT3 in the epilepsy rat caused by penicillin .Methods:The model of epilepsy in rats was made by abdominal injecting penicillin .Then the EEG and behavior was observed in each group and the level of IL-6/STAT3 transcription and cytokine IL-6 secretion in different groups was compared using qRT-PCR and ELISA respectively.The protein expression of STAT3 was detected using Western blot.Results: 500 U/kg and 800 U/kg rats injected penicillin were induced epilepsy and show typical epileptic seizures behavioral and brain waves ,seizures was 100%;the level of IL-6/STAT3 transcription and IL-6 secretion in the model of epilepsy was significantly higher than control .There was no significant difference in the expression of STAT3 protein levels,but a significant increase in p-STAT3.Conclusion:Expression of STAT3 and IL-6 plays an important role in the Epilepsy Rat caused by penicillin .
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Objective To approach on the expression pattern of MMP-2 and E-Cadherin in the effects of gastrin on gastric canc-er cells ,so as to investigate their role in the metastasis and invasion of gastric cancer .Methods Genomic DNA modified by sodium bisulfite was used as a template .RQ-MSP detect the methylation status of the gene promoter .The relative amount of Methylation status was performed with comparative threshold cycle (2- △ t ) method .Results MMP-2 and E-Cadherin genes in gastric cancer cells were at hemimethylated status in gastrin before and after the intervention .Gastrin can reduce the methylation levels of MMP-2 gene(P0 .05) .Gastrin can pro-mote the methylation levels of MMP-2 gene ,but proglumide decrease that effect (P<0 .05) .Conclusion Gastrin plays a certain role in the biological behavior of gastric cancer impact by changeing on the expression pattern of MMP-2 and E-Cadherin in the effects of gastrin on gastric cancer cells .
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Background and purpose:Because of the heterogeneity of cancers,different patients with the same kind of cancer have different sensitivity for cytotoxic drugs.Newly developed ATP chemosensitivity assays(ATPTCA) offer the opportunity for individualized therapy and have shown promising results compared to standard regimens.We explored the feasibility of the ATP-Tumor Chemosensitivity Assay(ATP-TCA) applied in the treatment of ovarian cancer with chemotherapy,and evaluate the in vitro sensitivity of fresh specimens of ovarian cancer to five cytotoxic drugs.Methods:ATP-TCA was used to detect the sensitivity rate of 24 fresh specimens of ovarian cancer to five cytotoxic drugs as follows: paclitaxel(TAX),gemcitabine(GEM),doxorubicin(ADM),topotecan(TPT),cisplatin(DDP).Results:23 of 24 specimens((95.83%)) can be evaluated by ATP-TCA,the sensitive rates of ATP-TCA was 91.30% for TAX,86.96% for GEM,65.22% for ADM,47.83% for TPT and 21.74% for DDP,respectively.Conclusions:ATP-TCA was a sensitive、reliable and standardized method for the evaluation of tumor chemosensitivity,it ts worthwhile to investigate further for the application screening anticancer agents in chemotherapy of ovarian cancer.